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KMID : 0811720010050030231
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Korean Journal of Physiology & Pharmacology
2001 Volume.5 No. 3 p.231 ~ p.242
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Extracellular ATP Stimulates Na+ and Cl- Transport through the Activation of Multiple Purinergic Receptors on the Apical and Basolateral Membranes in M-1 Mouse Cortical Collecting Duct Cells
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Jin Sup Jung/Jin Sup Jung
Sook Mi Hwang/Ryang Hwa Lee/Soo Kyung Kang/Jae Suk Woo/Yong Keun Kim
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Abstract
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The mammalian cortical collecting duct (CCD) plays a major role in regulating renal NaCl reabsorption, which is important in Na+ and Cl? homeostasis. The M-1 cell line, derived from the mouse cortical collecting duct, has been used as a mammalian model of the study on the electrolytes transport in CCD. M-1 cells were grown on collagen-coated permeable support and short circuit current (Isc) was measured. M-1 cells developed amiloride-sensitive current 5∼7 days after seeding. Apical and basolateral addition of ATP induced increase in Isc in M-1 cells, which was partly retained in Na+?free or Cl??free solution, indicating that ATP increased Na+ absorption and Cl? secretion in M-1 cells. Cl? secretion was mediated by the activation of apical cystic fibrosis transmembrane regulator (CFTR) chloride channels and Ca2+?activated chloride channels, but Na+ absorption was not mediated by activation of epithelal sodium channel (ENaC). ATP increased cAMP content in M-1 cells. The RT-PCR analysis demonstrated that M-1 cells express P2Y2,P2X3andP2Y4 receptors. These results showed that ATP regulates Na+ and Cl? transports via multiple P2 purinoceptors on the apical and basolateral membranes in M-1 cells.
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KEYWORD
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Cortical collecting duct cells, ATP, ATP, Purinergic receptors, Expression,
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